Supercooling preservation and transplantation of the rat liver
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چکیده
منابع مشابه
Supercooling as a Viable Non-Freezing Cell Preservation Method of Rat Hepatocytes
Supercooling preservation holds the potential to drastically extend the preservation time of organs, tissues and engineered tissue products, and fragile cell types that do not lend themselves well to cryopreservation or vitrification. Here, we investigate the effects of supercooling preservation (SCP at -4(o)C) on primary rat hepatocytes stored in cryovials and compare its success (high viabili...
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Piggyback orthotopic liver transplantation was performed in 24 patients during a period of 4 months. This represented 19% of the liver transplantation at our institution during that time. The piggyback method of liver insertion compared favorably with the standard operation in terms of patient survival, blood loss, incidence of vascular and biliary complications, and rate of retransplantation. ...
متن کاملCalpain is a mediator of preservation-reperfusion injury in rat liver transplantation.
Proteases as well as alterations in intracellular calcium have important roles in hepatic preservation-reperfusion injury, and increased calpain activity recently has been demonstrated in liver allografts. Experiments were designed to evaluate (i) hepatic cytosolic calpain activity during different periods of cold ischemia (CI), rewarming, or reperfusion, and (ii) effects of inhibition of calpa...
متن کاملPreservation solutions in liver transplantation: what are the options?
Alexis Carrel took the initial steps in the area of organ preservation by successfully preserving an artery for several days using chilled Locke’s solution, although this effort was largely overshadowed by his work in vascular surgery and organ transplantation. With the increased interest in clinical organ transplantation in the 1960s, the transplant community soon realized the need for preserv...
متن کاملNF-κB Induced the Donor Liver Cold Preservation Related Acute Lung Injury in Rat Liver Transplantation Model
BACKGROUND We have observed at our clinical work that acute lung injury (ALI) often occurs in patients transplanted with donor livers persevered for long time. So, we conducted this study to investigate the influence of cold preservation time (CPT) of donor liver on ALI induced by liver transplantation (LT), and further study the role of nuclear factor-κB (NF-κB) in the process. METHODS Wista...
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ژورنال
عنوان ژورنال: Nature Protocols
سال: 2015
ISSN: 1754-2189,1750-2799
DOI: 10.1038/nprot.2015.011